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研究者情報

 

基本情報

 
研究者名(漢字)
津下 英明
 
研究者名(カナ)
ツゲ ヒデアキ
 
研究者名(ローマ字)
TSUGE HIDEAKI
 
 

研究者基礎データ

 

研究職歴

1989 JT Life science institute Researcher
1994 Agouron Pharmaceviticals Inc. Researcher
1997 Tokushima Bunri University Associate Professor
2002 Tokushima Bunri University Professor
2010 Kyoto Sangyo University Professor
 

所属部署名

総合生命科学部
 

職名

教授 
 

ホームページURL

http://www.cc.kyoto-su.ac.jp/~tsuge/tsugelab/index.html 
 

出身大学院

北海道大学 北海道大学 理学部  理学研究科 理学 
 

出身学校

北海道大学 北海道大学 理学部 理学部 理学 
 

研究分野・キーワード

構造生物学、生物物理学
 

研究テーマ

1.新規蛋白質デザイン 新たなタンパク質こそのデザインを構造をもとに進めている。 構造生物化学 生物物理学  2005  

2.蛋白質複合体研究 タンパク質の複合体を捉え知る手段としてX線結晶構造解析を用いて研究を進めている。 構造生物化学 生物物理学  2000  

3.超好熱菌酵素の構造生物学 アーキアの代謝系酵素の構造生物学を進めている。 構造生物化学 生物物理学  2000  

4.ADPリボシル化毒素の構造生物学 細菌のモノADPリボシル化毒素とその基質タンパク質複合体の構造生物学研究を進めている。 構造生物化学 生物物理学  1999  

全て表示する(4件)

 

研究者活動データ

 

委員歴・役員歴

ビタミンB研究委員会 委員
日本学術振興会 回析構造生物第169委員会 委員
日本生化学会 評議員
 

取得学位名

博士(理学) 北海道大学 北海道大学 理学部 
 

学会

1.日本ビタミン学会   

2.日本蛋白質科学会   

3.日本生物物理学会 地区編集委員 2006 

4.日本結晶学会 編集委員 2006,2007,2008 

5.日本生化学会   

6.日本生物物理学会   

全て表示する(6件)

受賞学術賞

第45回 徳島新聞 科学賞 2009年 国内 
 

論文

1. Conformational plasticity is crucial for C3-RhoA complex formation by ARTT-loop Pathog Dis Tsuge H, Yoshida T, Tsurumura T.  73(9)  2015  

2. Rho GTPase Recognition by C3 Exoenzyme Based on C3-RhoA Complex Structure. J Biol Chem Toda A, Tsurumura T, Yoshida T, Tsumori Y, Tsuge H  290(32) 19423-32 2015  

3. Structural Basis for Action of the External Chaperone for a Propeptide-deficient Serine Protease from Aeromonas sobria. J Biol Chem. Kobayashi H, Yoshida T, Miyakawa T, Tashiro M, Okamoto K, Yamanaka H, Tanokura M, Tsuge H.  290(17) 11130-11143 2015  

4.10.1007/82_2014_415. Reaction Mechanism of Mono-ADP-Ribosyltransferase Based on Structures of the Complex of Enzyme and Substrate Protein. Curr Top Microbiol Immunol.  Tsuge H, Tsurumura T.  384 69-87 2015  

5.10.1007/s10969-014-9181-2.  Substrate selectivity of bacterial monoacylglycerol lipase based on crystal structure. J Struct Funct Genomics. Tsurumura T, Tsuge H.  15(3) 83-9 2014  

6.10.1107/S2053230X13032603. Crystallization and preliminary X-ray diffraction studies of a surface mutant of the middle domain of PB2 from human influenza A (H1N1) virus. Acta Crystallogr F Struct Biol Commun. Tsurumura T, Qiu H, Yoshida T, Tsumori Y, Tsuge H.  70(Pt 1) 72-5 2014  

7.10.1371/journal.pone.0082020. Conformational polymorphism of m7GTP in crystal structure of the PB2 middle domain from human influenza A virus. PLoS One Tsurumura T, Qiu H, Yoshida T, Tsumori Y, Hatakeyama D, Kuzuhara T, Tsuge H.  8(11)    

8. [The tertiary structures of the domains of influenza RNA polymerase]. Seikagaku Kuzuhara T, Tsuge H.  84(9) 780-5 2012  

9.10.1073/pnas.1217227110. Arginine ADP-ribosylation mechanism based on structural snapshots of iota-toxin and actin complex.


 Proc Natl Acad Sci U.S.A.  Tsurumura T, Qiu H. Tsumori Y, Oda M, Nagahama M, Sakurai J, Tsuge H   110(11): 4267-4272.  2013  

10.10.1016/j.febslet.2012.10.049. Crystal structures of dye-decolorizing peroxidase with ascorbic acid and 2,6-dimethoxyphenol.


 FEBS Lett. Yoshida T, Tsuge H, Hisabori T, Sugano Y.   586(24): 4351-6. 2012  

11. Role of sphingomyelinase in infectious diseases caused by Bacillus cereus  PLoS One.  Oda M, Hashimoto M, Takahashi M, Ohmae Y, Seike S, Kato R, Fujita A, Tsuge H, Nagahama M, Ochi S, Sasahara T, Hayashi S, Hirai Y, Sakurai J.   7(6)  2012  

12. Role of side-edge site of sphingomyelinase from Bacillus cereus.


 Biochem Biophys Res Commun. Oda M, Takahashi M, Tsuge H, Nagahama M, Sakurai J.   422(1): 128-32 2012  

13. Structure and stability of Gyuba, b-lactoglobulin chimera.


 Protein Sci.  Ohtomo H, Konuma T, Utsunomiya H, Tsuge H, Ikeguchi M.   20,  1867-75.  2011 

14. Structural basis of the free reduced flavin generation by flavin reductase from Thermus thermophilus HB8.

 J. Biol. Chem.  Imagawa T, Tsurumura T, Sugimoto Y, Aki K, Ishidoh K, Kuramitsu S, Tsuge H.  286,  44078-44085. 2011  

15. The catalytic mechanism of dye-decolorizing peroxidase DyP may require the swinging movement of an aspartic acid residue.


 FEBS J Yoshida T, Tsuge H, Konno H, Hisabori T, Sugano Y  278,  2387-2394. 2011  

16. Nucleolin as cell surface receptor for tumor necrosis factor-alpha inducing protein: a carcinogenic factor of Helicobacter pylori.  J Cancer Res Clin Oncol.  Watanabe T, Tsuge H, Imagawa T, Kise D, Hirano K, Beppu M, Takahashi A, Yamaguchi K, Fujiki H, Suganuma M.   136(6): 911-21 2010  

17. The carboxy-terminal tail of Aeromonas sobria Serine Protease is associated with the chaperone.  Microbiol Immunol.  Kobayashi H, Tateishi A, Tsuge H, Takahashi E, Okamoto K, Yamanaka H.   53(12): 647-57. 2009  

18. Structural basis for the kexin-like serine protease from Aeromonas sobria as sepsis-causing factor.  J Biol Chem.  Kobayashi H, Utsunomiya H, Yamanaka H, Sei Y, Katunuma N, Okamoto K, Tsuge H.   284(40): 27655-63 2009  

19. Structural basis for the Helicobacter pylori-carcinogenic TNF-alpha-inducing protein.  Biochem Biophys Res Commun.  Tsuge H, Tsurumura T, Utsunomiya H, Kise D, Kuzuhara T, Watanabe T, Fujiki H, Suganuma M.   388(2) 193-8. 2009  

20. Refolding, characterization and crystal structure of (S)-malate dehydrogenase from the hyperthermophilic archaeon Aeropyrum pernix.  Biochim Biophys Acta.  Kawakami R, Sakuraba H, Goda S, Tsuge H, Ohshima T.   1794(10): 1496-504 2009  

21. Structural basis of the influenza A virus RNA polymerase PB2 RNA-binding domain containing the pathogenicity- Determinant lysine 627 residue.  J Biol Chem.  Kuzuhara T, Kise D, Yoshida H, Horita T, Murazaki Y, Nishimura A, Echigo N, Utsunomiya H, Tsuge H.    284(11): 6855-60. 2009  

22. Crystallization and X-ray diffraction analysis of RNA-primer/promoter-binding domain of influenza A virus RNA-dependent RNA polymerase PB2.  Acta Crystallogr Sect F Struct Biol Cryst Commun.  Kuzuhara T, Kise D, Yoshida H, Horita T, Murazaki Y, Utsunomiya H, Tsuge H.   65: 144-6.  2009  

23. Non-native alpha-helix formation is not necessary for folding of lipocalin:Comparison of burst-phase folding between tear lipocalin and beta-lactoglobulin.  Proteins  Tsukamoto S, Yamashita T, Yamada Y, Fujiwara K, Maki K, Kuwajima K, Matsumura Y, Kihara H, Tsuge H, Ikeguchi M.   76(1): 226-36. 2008  

24. Structural analysis of human glutamine:fructose-6-phosphate amidotransferase, a key regulator in type 2 diabetes.  FEBS Lett.  Nakaishi Y., Bando M., Shimizu H., Watanabe K., Goto F., Tsuge H., Kondo K. and Komatsu M.   583(1): 163-7. 2008  

25. Structure of an archaeal alanine : glyoxylate aminotransferase.  Acta Crystallogr D Biol Crystallogr.  Sakuraba H., Yoneda K., Takeuchi K., Tsuge H., Katunuma N. and Ohshima T.   64(6): 696-699 2008  

26. Structural basis of actin recognition and arginine ADP-ribosylation by Clostridium perfringens iota-toxin.   Proc Natl Acad Sci U.S.A.  Tsuge H.*, Nagahama M., Oda M., Iwamoto S., Utsunomiya H., Victor E.M., Katunuma N., Nishizawa M. and Sakurai J.   105(21): 7399-7404.  2008  

27. Structure of 1-aspartate oxidase from the hyperthermophilic archaeon Sulfolobus tokodaii.  Biochim Biophys Acta.  Sakuraba H, Yoneda K, Asai I, Tsuge H, Katunuma N. and Ohshima T.   1784(3): 563-571.  2008  

28.  Crystal structure of the YdjC-family protein TTHB029 from Thermus thermophilus HB8:Structural relationship with peptidoglycan N-acetylglucosamine deacetylase.  Biochem Biophys Res Commun.  Imagawa T., Iino H., Kanagawa M., Ebihara A., Kuramitsu S. and Tsuge H.*.   367(3): 535-541. 2008  

29. Human D-amino acid oxidase: an update and review.  Chem Rec. Kawazoe T., Park HK., Iwana S., Tsuge H. and Fukui K.   7(5): 305-315.  2007  

30. Sequential aldol condensation catalyzed by hyperthermophilic 2-deoxy-d-ribose-5-phosphate aldolase.  Appl Environ Microbiol.  Sakuraba H., Yoneda K., Yoshihara K., Satoh K., Kawakami R., Uto Y., Tsuge H., Takahashi K., Hori H. and Ohshima T.   73(22): 7427-7434.  2007  

31. Crystal structure of archaeal highly thermostable L-aspartate dehydrogenase/NAD/citrate ternary complex. FEBS Journal.  Yoneda K., Sakuraba H., Tsuge H., Katunuma N. and Ohshima T.   274(16) 4315-4325.    

32. Structural basis of D-DOPA oxidation by D-amino acid oxidase:alternative pathway for dopamine biosynthesis.  Biochem Biophys Res Commun.  Kawazoe T., Tsuge H., Imagawa T., Aki K., Kuramitsu S. and Fukui K.   355(2): 385-391 2007  

33. Crystal structure of human D-amino acid oxidase: context-dependent variability of the backbone conformation of the VAAGL hydrophobic stretch located at the si-face of the flavin ring. Protein Science. Kawazoe T., Tsuge H., Pilone MS. and Fukui K.    15(12): 2708-2717. 2006  

34. Structure of a Hyperthermophilic Archaeal Homing Endonuclease (HEase), I-Tsp061I: Contribution of Cross-domain Polar Networks to Thermostability.  J Mol Biol.  Nakayama H., Shimamura T., Imagawa T., Shirai N., Itoh T., Sako Y., Miyano M., Sakuraba H., Ohshima T., Nomura N. and Tsuge H.*  365(2): 362-378.  2007  

35. Structural basis of the sphingomyelin phosphodiesterase activity in neutral sphingomyelinase from bacillus cereus. J Biol Chem.  Ago H., Oda M., Takahashi M., Tsuge H., Ochi S., Katunuma N., Miyano M. and Sakurai J.   281(23) 16157-16167.  2005  

36. Crystal structure of a novel FAD-, FMN-, and ATP-containing L-Proline Dehydrogenase Complex from Pyrococcus horikoshii.  J Biol Chem. Tsuge H.*, Kawakami R., Sakuraba H., Ago H., Miyano M., Aki K., Katunuma N. and Ohshima T.   280(35) 31045-31049. 2005  

37. A second novel dye-linked L-proline dehydrogenase complex is present in the hyperthermophilic archaeon Pyrococcus horikoshii OT-3. FEBS Journal.  Kawakami R., Sakuraba H., Tsuge H., Goda S., Katunuma N. and Ohshima T.  272(16) 4044-4054.  2005  

38. Crystal structure of the NAD biosynthetic enzyme quinolinate synthase. J Biol Chem.  Sakuraba H., Tsuge H., Yoneda K., Katunuma N. and Ohshima T.   280(29) 26645-26648 2005  

39. The first crystal structure of hyperthermostable NAD-dependent glutamate dehydrogenase from Pyrobaculum islandicum. J.Mol.Biol.  Bhuiya M.W., Sakuraba H., Ohshima T., Imagawa T., Katunuma N. and Tsuge H.*  345(2) 325-337 2005
  

40. The first crystal structure of an archaeal helical repeat protein.  Acta Cryst. F Yoneda K., Sakuraba H., Tsuge H., Katunuma N., Kuramitsu S., Kawabata T. and Ohshima T.  61(7) 636-639. 2005  

41. Amino-Acid Residues Involved in the Expression of the Activity of Escherichia coli TolC. Microbiol. Immunol.  Yamanaka H., Morisada N., Miyano M., Tsuge H., Shinoda S., Takahashi E. and Okamoto K.   48(10) 713-722 2004  

42. Crystallization and preliminary X-ray diffraction analysis of homing endonuclease Ⅰ-Tsp061Ⅰ.  Acta Crystallogr D Biol Crystallogr.  Imagawa T., Nakayama H., Katunuma N., Sakuraba H., Ohshima T., Itoh T., Sako Y., Nomura N. and Tsuge H.*    60(11) 2006-2008.  2004  

43. Crystallization and preliminary X-ray diffraction analysis of the hyperthermostable NAD-dependent glutamate dehydrogenase from Pyrobaculum islandicum. Acta Crystallogr D Biol Crystallogr. Bhuiya M.W., Sakuraba H., Yoneda K., Ohshima T., Imagawa T., Katunuma N. and Tsuge H.*   60(4) 715-717 2004  

44. Crystal structure and site-directed mutagenesis of enzymatic components from Clostridium perfringens iota-toxin.
 J Mol Biol. Tsuge H.*, Nagahama M., Nishimura H., Hisatsune J., Sakaguchi Y., Itogawa Y., Katunuma N. and Sakurai J.  325(3),471-483/,   2003  

45. The first crystal structure of archaeal aldolase. Unique tetrameric structure of 2-deoxy-d-ribose-5-phosphate aldolase from the hyperthermophilic archaea Aeropyrum pernix.
 J Biol Chem. Sakuraba H., Tsuge H., Shimoya I., Kawakami R., Goda S., Kawarabayasi Y., Katunuma N., Ago H., Miyano M. and Ohshima T. 278(12),10799-10806/,   2003  

46. Clostridium perfringens iota-toxin, ADP-ribosyltransferase: structure and mechanism of action.
 Adv Enzyme Regul. Sakurai J., Nagahama M., Hisatsune J., Katunuma N. and Tsuge H. 43,361-377/,   2003  

47. Identification of 16 novel mutations in the argininosuccinate synthetase gene and genotype-phenotype correlation in 38 classical citrullinemia patients. Hum Mutat. Gao H.Z., Kobayashi K., Tabata A., Tsuge H., Iijima M., Yasuda T., Kalkanoglu H.S., Dursun A., Tokatli A., Coskun T., Trefz F.K., Skladal D., Mandel H., Seidel J., Kodama S., Shirane S., Ichida T., Makino S., Yoshino M., Kang J.H., Mizuguchi M., Barshop B.A., Fuchinoue S., Seneca S., Zeesman S., Knerr I., Rodes M., Wasant P., Yoshida I., De Meirleir L., Abdul Jalil M., Begum L., Horiuchi M., Katunuma N., Nakagawa S. and Saheki T. 22(1),24-34/,   2003  

48. Crystal structure of the ADP-dependent glucokinase from Phrococcus horikoshii at 2.0-A resolution:a large conformational change in ADP-dependent glucokinase. Protein Sci. Tsuge H.*, Sakuraba H., Kobe T., Kujime A., Katunuma N. and Ohshima T.  11(10), 2456-2463/,   2002  

49. Crystallization and preliminary X-ray diffraction analysis of glutamate dehydrogenase from an aerobic hyperthermophilic archaeon, Aeropyrum pernix K1.
 Acta Crystallogr D Biol Crystallogr. Bhuiya M.W., Tsuge H., Sakuraba H., Yoneda K., Katunuma N.and Ohshima T. 58(Pt8), 1338-1339/,   2002  

50. Crystallization and preliminary X-ray crystallographic studies of monoacylglycerol lipase of the moderately thermophilic Bacillus sp. H-257. Acta Crystallogr D Biol Crystallogr. Yoneda K., Nishimura T., Katunuma N., Imamura S., Nitta K. and Tsuge H. 58(Pt7), 1232-1233/,   2002  

51. Chondroitin sulfate proteoglycan is a potent enhancer in the processing of procathepsin L. Biol Chem. Kihara M., Kakegawa H., Matano Y., Murata E., Tsuge H., Kido H. and Katunuma N.  383(12),1925-1929/,   2002  

52. Structure-based development of cathepsin L inhibitors and therapeutic applications for prevention of cancer metastasis and cancer-induced osteoporosis.
 Adv Enzyme Regul. Katunuma N., Tsuge H., Nukatsuka M. and Fukushima M.  42,159-172/,   2002  

53. Structure-based design of specific cathepsin inhibitors and their application to protection of bone metastases of cancer cells.
 Arch Biochem Biophys. Katunuma N., Tsuge H., Nukatsuka M., Asao T. and Fukushima M.  397(2),305-311/,   2002  

54. カテプシンL選別的阻害剤のStructure Based Drug Design 法による開発 生物工学  78巻10号 421-424/,   2000  

55. Inhibition mechanism of cathepsin L-specific inhibitors based on the crystal structure of papain-CLIK148 complex. Biochem Biophys Res Commun. Tsuge H.*, Nishimura T., Tada Y., Asao T., Turk D., Turk V. and Katunuma N. 266(2), 411-416/,   1999  

56. Structure based development of novel specific inhibitors for cathepsin L and cathepsin S in vitro and in vivo.
 FEBS Lett. Katunuma N., Murata E., Kakegawa H., Matsui A., Tsuzuki H., Tsuge H., Turk D., Turk V., Fukushima M., Tada Y. and Asao T.  458(1),6-10/,   1999  

57. Crystallization and preliminary X-ray studies of the Ia component of Clostridium perfringens iota toxin complexed with NADPH.
 J Struct Biol. Tsuge H.*, Nagahama M., Nishimura T., Sakaguchi Y., Katunuma N. and Sakurai J. 126(2),175-177/,   1999  

58. Structure of the Human Cytomegalovirus Protease Catalitic Domain Reveals a Novel Serine Protease Fold and Catalytic Triad.
 Cell. Chen P., Tsuge H., Almassy RJ., Gribskov CL., Katoh S., Vanderpool DL., Margosiak SA., Pinko C., Matthews DA. and Kan CC. 86  835-843 1996  

全て表示する(58件)

発表(口頭・ポスター)

1.DNAライブラリーから発現量の多い可用性領域を迅速に選択する手法の開発 日本生化学会、神戸ポートアイランド(神戸) 2015 12 ポスター 

2.Aeromonas sobria由来プロペプチド欠損プロテアーゼの外部シャペロンによるフォールディング機構 日本生化学会、神戸ポートアイランド(神戸) 2015 12 口頭発表 

3.ラン藻アナベナ由来 12量体グルタミン合成酵素の結晶構造  第53回日本生物物理学会、金沢大学(金沢) 2015 09 口頭発表 

4.Structural basis of ADP-ribosyltransferase C3 exoenzyme with RhoA complex 第53回日本生物物理学会、金沢大学(金沢) 2015 09 口頭発表 

5.ADPリボシル化酵素C3とRhoA複合体の構造とその認識機構 第441回ビタミンB研究協議会、ホテルコスモスクエア国際交流センター(大阪) 2015 09 口頭発表 

6.Structural basis of Bacterial ADP-ribosyltransferase and Human Protein Complex ETOX17(European Workshop on Bacterial Protein Toxins) (Braga) 2015 06 口頭発表 

7.ADPリボシル化RhoAの立体構造に基づく阻害機構 ビタミンB研究協議会、大阪工業大学うめきたナレッジセンター(大阪) 2014 11 口頭発表 

8.細菌感染症因子とホストタンパク質複合体の構造生物学 第7回共同利用・共同研究「酵素学研究拠点」シンポジウム 勝沼信彦名誉教授追悼記念講演会、徳島大学疾患酵素学研究センター(徳島) 2014 11 口頭発表 

9.ADPリボシル化RhoAの構造とそのシグナル伝達への影響 日本生化学会、京都国際会館(京都) 2014 10 ポスター 

10.タンパク質の構造の見方・使い方: 細菌毒素による翻訳後修飾の構造生物学 徳島大学薬学部大学院特論(徳島大学) 2014 07 口頭発表 

11.Dye-decolorizing peroxiadseの触媒サイクルと基質結合部位 第61回日本生化学会 近畿支部年会 2014 05 口頭発表 

12.ウェルシュ菌毒素IaによるactinのADPリボシル化機構 第61回日本生化学会 近畿支部年会 2014 05 口頭発表 

13.Visualization of actin mono-ADP-ribosylation in iota toxin and actin complex structure. ASBMB annual meeting (SanDiego),  2014 04 ポスター 

全て表示する(13件)